Central base pair flipping and discrimination by PspGI
نویسندگان
چکیده
منابع مشابه
Central base pair flipping and discrimination by PspGI
PspGI is a representative of a group of restriction endonucleases that recognize a pentameric sequence related to CCNGG. Unlike the previously investigated Ecl18kI, which does not have any specificity for the central base pair, PspGI prefers A/T over G/C in its target site. Here, we present a structure of PspGI with target DNA at 1.7 A resolution. In this structure, the bases at the center of t...
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The PspGI restriction-modification system recognizes the sequence CCWGG. R.PspGI cuts DNA before the first C in the cognate sequence and M.PspGI is thought to methylate N4 of one of the cytosines in the sequence. M.PspGI enhances fluorescence of 2-aminopurine in DNA if it replaces the second C in the sequence, while R.PspGI enhances fluorescence when the fluorophore replaces adenine in the cent...
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Although change and adaptation are key to life, nature is reluctant to abandon old inventions. Even today when the hypothetical RNA world of our ancestors has been supplanted by the richer possibilities offered by proteins, we still find key processes catalyzed by RNA. Perhaps the oldest discovery was the unique power of base pairing to enable replication. This must have been key to the develop...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2008
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkn622